Plant analysis

Plant analysis

In addition to phytopathology and herbology, IDENTXX has continuously expanded its services in plant analysis.
Discover the possibilities of modern molecular biology for your crop production.

dna

Species determination

In some cases the phenotype is not indicative of the species. Or there are no features such as leaves or flowers.

In these cases, molecular species identification is the right analytical tool for your problem.

chromosomes

Marker-based selection

In addition to traditional phenotypic selection, modern plant breeding relies on molecular methods.

IDENTXX supports vine breeders with the analysis of genetic markers for the development of fungus-resistant vines.

genetics

Epigenetics

One speaks of epigenetics when external influences have a lasting effect on the reading of genes without changing the base sequence.

This is based on the methylation of individual DNA building blocks, which the IDENTXX can analyze for you.

dna

Species determination

In some cases the phenotype is not indicative of the species. Or there are no features such as leaves or flowers.

In these cases, molecular species identification is the right analytical tool for your problem.

chromosomes

Marker-based selection

In addition to traditional phenotypic selection, modern plant breeding relies on molecular methods.

IDENTXX supports vine breeders with the analysis of genetic markers for the development of fungus-resistant vines

genetics

Epigenetics

One speaks of epigenetics when external influences have a lasting effect on the reading of genes without changing the base sequence.

The basis for this are methylations of individual DNA building blocks, which the IDENTXX can analyze for you.

Our processing time is usually 10 working days.

Fields of use species identification

Molecular biological species identification enables classification when the p notype is unclear or not enough tissue is available for a classic identification. Use this advantage e.g. B. in the following applications:

Ginkgo biloba

The female flowers or the fruits of Ginkgo biloba produce buttery acid, which we humans find unpleasant. However, the species develops its flowers very late (> 20 years), which can lead to problems, especially in public green spaces.

With the sex-differentiating PCR from IDENTXX, you have the opportunity to quickly, easily and inexpensively get clarity about your ginkgo tree.

Root examination

If roots cause damage to private or public structures, the question of the causative plants arises. If this is not recognizable at first glance, molecular species identification can help. This not only saves you the expensive tracing back through excavations, it is also much faster.

sighting of the samples
Hybrids

The hybrids of two species can in some cases be visually indistinguishable from their parents.

In this case, too, the sequencing of certain gene segments can provide information about family relationships in the examined sample.

Sampling process ? Species determination

Molecular species determination can be carried out using different strategies. We determine which test setup is the right one for your purposes in a non-binding preliminary discussion.

Joint determination of the sampling strategy

Sample receipt

DNA extraction

Standard processing time is 10 working days from sample receipt

Areas of application Marker-based selection

The breeding of new varieties is the key factor for secure plant production. It often takes years of intensive breeding work from the first crossing to the marketable new variety. IDENTXX accompanies your development process with molecular biological analyses. Follow how individual genes or markers become established in the offspring. We only need small amounts of leaves for the analyses, which enables the selection of suitable offspring at an early stage of development. This not only saves costs, but can significantly speed up the entire breeding process.

Vitis vinifera (grape vine)

Fungus-resistant vines, called PIWIs for short, make it possible to significantly reduce the application of fungicides or even to do without them altogether. A real benefit for the environment and therefore also for people.
Intensive research work at universities and state institutions made it possible to identify genetic markers that are inherited together with the resistance characteristics. With the help of fragment length analysis, these sections of genetic material can be precisely determined and evaluated.

What can be examined

Based on our experience, it is possible to examine all types of tissue, from leaf to wood, everything is possible. The younger and fresher the material, the faster the examination can be carried out.

Working on the vines
Available resistance markers of the vine

We can currently analyze the following resistance markers for you:

Sampling process ? Marker-based selection

Joint determination of the sampling strategy

Sample receipt

DNA extraction

The exact length of the fragment is determined with the help of fluorescence-marked PCR products in a high-throughput process

Evaluation

Field of application epigenetics

In plant breeding, the use of epigenetic information is an innovative approach. New properties are created by switching genes on or off without changing the plant's fundamental genetic code.

DNA methylation

The basic process behind epigenetics is the methylation of certain DNA building blocks. The most important methylation process for analysis is the modification of cytosine to methylcytosine.

Analysis method

A combination of different working techniques is used to determine methylation patterns. After DNA extraction, the genome is first subjected to a bisulfite reaction. In the process, unmethylated cytosines are converted to uracils, while the methylcytosines remain unchanged. A qualitative PCR that is then carried out in combination with the pyrosequencing method then makes it possible to determine the methylation pattern of the desired DNA segment.

DNA research
What can be examined

Based on our experience, it is possible to examine all types of tissue, from leaf to root, everything is possible.

For whom is the method suitable?

You are a plant breeder and have information about possible epigenetically modified breeding lines and want to follow them up in your further selection process.

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