In addition to phytopathology and herbology, IDENTXX has continuously developed its plant analysis capabilities.
Discover the potential of modern molecular biology for your plant production.
In some cases, the phenotype does not allow conclusions to be drawn about the species. Or characteristics such as leaves or flowers are not present.
In these cases, molecular species identification is the right analytical tool for your question.
In addition to traditional phenotypic selection, modern plant breeding relies on molecular methods.
IDENTXX supports grapevine breeders with the analysis of genetic markers for the development of fungus-resistant grapevines.
Epigenetics refers to the phenomenon where external influences have a lasting effect on the reading of genes without altering the base sequence.
This is based on the methylation of individual DNA building blocks, which IDENTXX can analyze for you.
In some cases, the phenotype does not allow conclusions to be drawn about the species. Or characteristics such as leaves or flowers are not present.
In these cases, molecular species identification is the right analytical tool for your question.
In addition to traditional phenotypic selection, modern plant breeding relies on molecular methods.
IDENTXX supports grapevine breeders with the analysis of genetic markers for the development of fungus-resistant grapevines
Epigenetics refers to the phenomenon where external influences have a lasting effect on the reading of genes without altering the base sequence.
This is based on methylation of individual DNA building blocks, which IDENTXX can analyze for it.
Breeding new varieties is the key to ensuring reliable plant production. From the initial crossbreeding to a marketable new variety, years of intensive breeding work often pass. IDENTXX accompanies your development process with molecular biological analyses. Track how individual genes or markers become established in the offspring. We require only small amounts of leaf samples for the analyses, which allows for the selection of suitable offspring at an early stage of development. This not only saves costs but can also significantly accelerate the entire breeding process.
Fungus-resistant grapevines, or PIWIs for short, make it possible to significantly reduce or even completely eliminate fungicide applications. This is a real benefit for the environment and therefore also for people.
Intensive research at universities and government institutions has identified genetic markers that are inherited along with resistance traits. Fragment length analysis allows these genetic segments to be precisely determined and evaluated.
Based on our experience, we can examine all types of tissue, from leaves to wood. The younger and fresher the material, the faster the examination can be carried out.
We can currently analyze the following resistance markers for you:
Joint determination of the sampling strategy
Sample receipt
DNA extraction
Qualitative PCR
Fragment length analysis
Evaluation
Molecular biological species identification allows for classification when the phenotype is unclear or insufficient tissue is available for classical identification. Utilize this advantage, for example, in the following applications:
The female flowers and fruits of Ginkgo biloba produce butyric acid, which humans find unpleasant. However, this species only flowers very late (over 20 years), which can cause problems, especially in public green spaces.
With IDENTXX's sex-differentiating PCR, you have the opportunity to quickly, easily, and cost-effectively obtain clarity about your Ginkgo tree.
When roots cause damage to private or public structures, the question arises as to which plants are responsible. If this is not immediately apparent, molecular species identification can help. This not only saves you the expensive process of tracing the plant's origin through excavation, but is also significantly faster.
In some cases, hybrids of two species cannot be visually distinguished from their parents.
In this case too, sequencing certain gene segments can provide information about the kinship relationships of the sample under investigation.
Molecular species identification can be performed using various strategies. We will determine which test setup is right for your needs in a free initial consultation.
Joint determination of the sampling strategy
Sample receipt
DNA extraction
Qualitative PCR
Sanger sequencing
Standard processing time: 10 working days from receipt of sample
In plant breeding, the use of epigenetic information is an innovative approach. New traits arise by switching genes on or off without altering the plant's fundamental genetic code.
The fundamental process behind epigenetics is the methylation of certain DNA building blocks. The most important methylation process for analysis is the modification of cytosine to methylcytosine.
To determine methylation patterns, we used a combination of different techniques. After DNA extraction, the genome first undergoes a bisulfite reaction. This converts unmethylated cytosines to uracils, while methyl cytosines remain unchanged. Subsequent qualitative PCR, combined with pyrosequencing, then allows us to determine the methylation patterns of the desired DNA segment.
Based on our experience, it is possible to examine all types of tissue; everything is possible, from leaves to roots.
You are a plant breeder and have information about possible epigenetically modified breeding lines and want to pursue these further in your selection process.
contact
Address
information